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For western blot analysis of anti-FLAG immunoprecipitation, the beads were eluted with 30 µL of 3×FLAG peptide (150 ng/µL) in TBS for 30 min on a rotating wheel at 4 °C

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Regulation of FASN and de novo fatty-acid synthesis pathway in cancer The increased de novo fatty-acid synthesis is caused by multiple mechanisms, including increased expression of lipogenic enzymes